- DETECTX CORTICOSTERONE ENZYME IMMUNOASSAY KIT PDF PDF
- DETECTX CORTICOSTERONE ENZYME IMMUNOASSAY KIT PDF SOFTWARE
- DETECTX CORTICOSTERONE ENZYME IMMUNOASSAY KIT PDF FREE
DETECTX CORTICOSTERONE ENZYME IMMUNOASSAY KIT PDF SOFTWARE
The concentration of the 8-hydroxy-2’-deoxyguanosine in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers. After the reaction is stopped, the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength.
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The substrate reacts with the peroxidase- labeled antibody that has reacted with the bound conjugate. After a 2 hour incubation, the plate is washed and substrate added. The binding reaction is initiated by the addition of a peroxidase-labeled mouse monoclonal antibody to 8-hydroxy-2’-deoxyguanosine to each well. An 8-hydroxyguanosine conjugate is added to the standards and samples in the wells. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An 8-hydroxy-2’-deoxyguanosine (8-OHdG) stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Please read the complete kit insert before performing this assay. These species may be present in serum, plasma, saliva, urine, dried fecal samples, and tissue culture media samples. The assay detects all three oxidized guanine species 8-hydroxy-2’-deoxyguanosine from DNA, 8-hydroxyguanosine from RNA and 8-hydroxyguanine, from digested DNA from DNA or RNA. During the repair of damaged DNA in vivo by exonucleases, the resulting 8-OHdG is excreted without further metabolism into urine.ģ K059-H WEB 210302 ASSAY PRINCIPLE The DetectX® DNA Damage Immunoassay Kit is designed to quantitatively measure DNA and RNA oxidized guanosine species. 8-OHdG is physiologically formed and enhanced by chemical carcinogens. Among numerous types of oxidative DNA damage, the formation of 8-hydroxy-2’-deoxyguanosine (8-OHdG) is a ubiquitous marker of oxidative stress. It is widely thought that continuous oxidative damage to DNA is a significant contributor to the age-related development of the major cancers, such as those of the colon, breast, rectum, and prostate. Damage to RNA is reflected in nucleoside adducts. Deoxynucleotides are enzymatically hydrolyzed to stable deoxynucleosides, and these repair products are transported through the blood and excreted in the urine. When individual bases are damaged, nonspecific DNA repair enzymes excise DNA lesions to release deoxynucleotides, and base specific repair glycosylases excise the corresponding base. Cellular function may be interrupted or stopped if DNA damage corrupts the integrity of essential information contained in the genome.
DETECTX CORTICOSTERONE ENZYME IMMUNOASSAY KIT PDF FREE
Intracellular free radical species (ROS) are produced as a result of normal metabolism and extracellular forms are produced as a result of ultraviolet radiation or ionizing radiation. 11-13Ģ EXPECT ASSAY ARTISTRY™ K059-H WEB 210302 BACKGROUND Free radicals and other reactive species are constantly generated in vivo and cause oxidative damage to biomolecules, a process held in check only by the existence of multiple antioxidant and repair systems as well as the replacement of damaged nucleic acids, proteins and lipids. Validation Data Sensitivity, Linearity, etc. Please read this insert completely prior to using the product. Serum, EDTA and Heparin Plasma, Saliva, Urine, Digested DNA, Fecal Extracts and Tissue Culture Media The most sensitive, specific and flexible alternative to more costly and time-consuming methods for oxytocin detection.Įasy-to-use, well-validated serotonin ELISA kit for hormone research.Detectx® DNA Damage Enzyme Immunoassay KitĭetectX® DNA Damage Enzyme Immunoassay Kitġ Plate Kit Catalog Number K059-H1 5 Plate Kit Catalog Number K059-H5 Widely used sensitive cortisol ELISA kit with a large variety of sample types and highly reproducible results for stress, autoimmune disease and cancer research.
DETECTX CORTICOSTERONE ENZYME IMMUNOASSAY KIT PDF PDF
Highly sensitive corticosterone ELISA kit, species independent, used for animal stress research.ĮLISA, Colorimetric detection | Print as PDF This product is compatible with the Absorbance 96 Plate Reader.
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Cited sample types include cell lysate, urine and whole blood.Ĭorticosterone (100%), Deoxycorticosterone (21.3%), Desoxycorticosterone (21.0%), Progesterone (0.46%), Testosterone (0.31%), Tetrahydrocorticosterone (0.28%), Aldosterone (0.18%), Cortisol (0.046%) and 5 minutes before diluting with EIA buffer.Īdd 380µl EIA assay buffer to each plasma tube, vortex.įinal dilution is ~1:40 (This final dilution may not be appropriate for every sample thus, optimal dilution must be determined by each end user for their experimental sample group.) For the quantitative determination of Corticosterone in culture supernatants, feces, plasma, serum, and saliva from any species.